Fig. 2. WWOX-overexpressing BCC cells secrete bFGF to support microtubule formation by HUVEC cells.

a The condition media (CM) from the stable transfectants of BCC cells, as described above, were used to carry out CAM assay using chick embryos. b Endogenous WWOX and ectopic EGFP and EGFP-WWOX proteins are shown in the stable transfectants. c, d Expression of bFGF protein is shown in the BCC stable transfectants with EGFP and EGFP-WWOX cDNA constructs. The level of bFGF protein in the EGFP-WWOX-expressing BCC is almost double that EGFP cells. e EGFP-WWOX-expressing BCC secreted greater amounts of bFGF protein than EGFP cells, as determined by ELISA. f The CM from the EGFP-WWOX-expressing BCC supported the tube formation of HUVEC cells. The serum-free medium and the CM form EGFP-expressing BCC had no effect. g, h The effect of serum on the production of bFGF is shown in EGFP and EGFP-WWOX-expressing BCC cells