Fig. 1.

Traj33 knockout (KO) mice have heavily reduced mucosal-associated invariant T (MAIT) cells a Flow cytometric analysis of MR1-5-OP-RU tetramer⁺ TCRβ⁺ MAIT cells from thymus and anti-CD24-complement-depleted enriched thymus, spleen, lymph node, lung and liver from Traj33 wild-type (WT), Traj33 heterozygous (het) and Traj33 KO mice. Plots were pre-gated on B220⁻ live lymphocytes, hence numbers show the percentage of gated MAIT cells of B220⁻ cells. b Frequencies of all T cells and absolute numbers of MAIT cells from the aforementioned organs in the respective mice. Horizontal bars on scatter points signify mean ± SEM. Each scatter point represents an individual mouse, and data are derived from three independent experiments with a total of eight WT, eight Traj33 het and eight Traj33 KO mice. Source data are provided as a source data file. Open symbols represent ex-breeders, >20 weeks old. Statistical significance is based on *P ≤ 0.05, and **P ≤ 0.01 using Mann–Whitney rank-sum U test with a Bonferroni correction for three comparisons