Fig. 3. Estrogen suppresses the expression of ENaC in mpkCCDc14 cells.

a Endogenous α-EnaC expression was dose-dependently decreased by E₂ treatment in mpkCCDc14 cells, as determined by western blot analysis. b Quantitation of the western blot data for the EnaC protein from a. The bars are the means ± SE values from three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the Con group. c E₂ suppressed the protein expression of α-ENaC in mpkCCDc14 cells during a time course, as detected by western blot analysis. d The mean time-course data for α-ENaC expression in C normalized to α-tubulin expression. The bars are the means ± SE values from three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the Con group. e Typical traces of short-circuit current (Isc, μA/cm²) across mpkCCDc14 cell epithelia under E₂ treatment at a series of concentrations. The arrows indicate the time of addition of 10 μM amiloride to the apical bath. f Amiloride-sensitive Isc from all experiments of the type shown in (e); the data are from four epithelia studied under each condition. *P < 0.05 compared to the Con group