Fig. 6. Estrogen induces α-ENaC ubiquitination by promoting the interaction of α-ENaC with derlin-1.

a The interaction between α-ENaC and derlin-1 was detected through co-IP analysis in mpkCCDc14 cells with or without E₂ stimulation. b Quantification of the binding signals from (a). c Double immunofluorescence staining was performed to confirm the interactions between α-ENaC and derlin-1 in mpkCCDc14 cells with or without E₂ treatment. d More ubiquitin conjugated to α-ENaC was detected in cells treated with E₂ than in untreated cells. e Quantification of the ubiquitin conjugated to α-ENaC shown in d normalized to β-actin expression