Fig. 8. Estrogen evokes phosphorylation of AMPK in mpkCCDc14 cells.

a The expression levels of phosphorylated AMPK and total AMPK in mpkCCDc14 cells stimulated with a series of E₂ concentrations were determined by western blot analysis. b Quantification of the phosphorylated AMPK expression shown in (a) normalized to β-actin expression. c The expression levels of phosphorylated AMPK and total AMPK in mpkCCDc14 cells during a time course of E₂ stimulation were determined by western blot analysis. d Quantification of the phosphorylated AMPK expression shown in c normalized to β-actin expression. The bars are the means ± SE values from three independent experiments. *P < 0.05 and **P < 0.01 compared to the Con group