Fig. 2. Immunization with DNA-pulsed dendritic cells induced SLE syndrome.

a DCs were induced from bone marrow cells with GM-CSF/IL-4 and were stained with isotype-control antibody (left panel) or anti-CD11c-PE (right panel). b BMDC-ALD-DNA or BMDC at 10⁶ cells were intravenously injected into B6 mice. The serum from each mouse were collected and anti-dsDNA Abs were measured by specific ELISA. c–e Ten weeks after BMDC-ALD-DNA immunization, kidney sections were stained by hematoxylin and eosin (400×) (c left), the renal score of two groups(c right), IgG immunohistochemically staining (400×) (d), complement 3 (400×) (e), Results are representatives of five mice. f Mice were immunized with ALD-DNA (50 μg/mouse) or BMDC-ALD-DNA at 10⁶ cells by intravenous injections. The serum from each mouse were collected every two weeks and the anti-dsDNA Abs were detected by specific ELISA