Fig. 5. Blockade of B7-H3 enhances DC activation.
a DC2.4 cells were stained with anti-B7-H3 mAb/APC (open) or control Ig (solid) and analyzed by flow cytometry. b DC2.4 cells at 5 × 105 were cultured with LPS (1ug/ml) for 24 h and subsequently with 14 M or control Ig at 5ug/ml for 24 h. Supernatants were collected for cytokine CBA. c After stimulation of DC2.4 by 0.005, 0.05, and 5μg/ml doses of 14 M or control Ig for 24 h, cells were intracellularly staining with an anti-phosphorylated STAT3 antibody. d Freshly prepared BMDC at 5 × 105 were cultured with LPS (1ug/ml) for 24 h and subsequently treated with plate-coated 14 M or control Ig at 5ug/ml for 24 h. Supernatants were collected for cytokine CBA