Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 21;9:7667. doi: 10.1038/s41598-019-43409-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TGFβ/Smad signaling pathway activation in response to TGFβ-stimulation. H1975, H1650 and H2030 cells were kept in serum-free medium overnight. Cells were stimulated with 2 ng/ml TGFβ and lysed at the indicated time points. Subsequently, Smad2/3 immunoprecipitation was followed by immunoblotting. (A) Immunoblotting data of H1975, H1650 and H2030 cells. One representative example is shown. (B) Treatment with TGFβR inhibitor SB-431542 (10 μM) completely abolishes Smad2/3 phosphorylation upon TGFβ-treatment. (C) TGFβ-induced reduction of cell deformability is abolished upon application of TGFβR inhibitor. H1975 cells were treated with 2 ng/ml TGFβ, in presence or absence of 10 μM SB-431542 inhibitor for 24 h. Trypsinized cells were injected into the microfluidic system of cell optical stretcher. At least 300 cells per condition were measured. n indicates number of independent repetitions.