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. 2019 May 21;9:7667. doi: 10.1038/s41598-019-43409-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamic response of MYH15, MYL9, MYLK and TPM1 to TGFβ-stimulation in NSCLC cell lines. (A) Single gene dynamics of candidates genes from the microarray. (B) Validation of the microarray candidate genes with qPCR in H1975 and H1650 cells. Cells were stimulated with 2 ng/ml TGFβ and RNA was extracted at the indicated time points. Gene expression was assessed using qRT-PCR. mRNA expression was normalized to the geometric mean of two housekeeper genes: G6PD and HPRT. *p < 0.05 and ***p < 0.001 significance levels were determined using one-way ANOVA. n indicates the number of independent repetitions.