Table 2.
PCR primers used in this study for RT-qPCR analysis of gene expression.
| Target locusa | Gene product (bp) | Gene description | F/R | Primer sequence (5′-3′) |
|---|---|---|---|---|
| V. parvula | ||||
| RS04165 | 126 | Glutamine synthase | F | GGCAATGCATTTGCTAAAGA |
| R | AATTGCAACCATGCTACGAA | |||
| RS04150 | 147 | Histidine triad protein | F | TGAAGGGTTACTGCCATTTG |
| R | GCCCATTTCTTCTCCACCTA | |||
| RS04540 | 89 | tRNA µ-54 methyltransferase | F | TCGGTGCGTTTATCCACTAA |
| R | ATGCCCGTAGAAATTATGGC | |||
| RS02475 | 89 | Sugar family | F | AATGGCCCGTAGAGGATATG |
| R | TTTCTCCATCAAATGAAGCG | |||
| RS00600 | 108 | Natural resistance-associated macrophage protein (NRAMP) | F | TTGAACAGGCTGAGGAGTTG |
| R | TACCTGCCGTAGCAGATGAG | |||
| 16S Vp | 108 | 16S rRNA (V. parvula) | F | CCGTGATGGGATGGAAACTGC |
| R | CCTTCGCCACTGGTGTTCTTC | |||
| S. gordonii | ||||
| RS09100 | 95 | Sucrose-6-phosphate hydrolase | F | GCCTGATGTGGAGTACCCTT |
| R | ATTGGTAGAGCTTGCCGTCT | |||
| RS06800 | 104 | Fe-S cluster assembly protein | F | GCCAGACTTTGGACCTGATT |
| R | CTTCAGGAACATCATCCCA | |||
| RS06955 | 94 | ABC transporter permease | F | TCGAAGTCAAGGGAAGTCCT |
| R | ATATAGGCAACCCGATCAGC | |||
| RS01315 | 124 | Transporter | F | GCTTGGACCTACTGGGTTGT |
| R | TCATTGGCATGGACAGACTT | |||
| RS04795 | 106 | Accessory secretory protein Asp5 | F | GGTGAAGACCGTCAAATCCT |
| R | TCTTACGTGGCTTGAGGTTG | |||
| RS07290 | 108 | β-galactosidase | F | GCTATTGCAGCAGATGGAAA |
| R | ATGCAATTGGAAACGAACAA | |||
| RS05725 | 127 | O family oxidase-dependent iron (Fe2+) transporter | F | TTGGCTCATTTATGCTCTGG |
| R | TGGATAAATTCCAGCCCAAT | |||
| RS08935 | 96 | d-Tyrosyl-tRNA deacylase | F | ACAAGCTCAGGTGTCGATTG |
| R | TCATCTGGACCATCATCAGG | |||
| RS02790 | 130 | Serine protease (Challisin) | F | ATCCGTCAGAGCAGGAGTCT |
| R | TCTCCTGCTGATGGAACTTG | |||
| RS00395 | 118 | Hypothetical protein | F | GCTCAAGCATGGCTAACAGA |
| R | CTTTAGCACCAGACCAAGCA | |||
| 16S Sg | 138 | 16S rRNA (S. gordonii) | F | AGACACGGCCCAGACTCCTAC |
| R | TCACACCCGTTCTTCTCTTACAA | |||
a‘HSIVP1’ numbers for V. parvula; ‘SGO’ numbers for S. gordonii.