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The HA titers of Bacillus subtilis 168 and WB600 cultured in shake flasks at 32 °C The strains are 1A (Bacillus subtilis 168, pHY300plk-hasA), 1B (Bacillus subtilis 168, pHY300plk-hasABC), WmA, and WmB, separately. 37 °C was used as a control temperature. Data are average of three independent experiments and error bars represent ± SD (*p < 0.05; **p < 0.01; ***p < 0.001)
