. 2019 May 21;9(6):225. doi: 10.1007/s13205-019-1749-x

Table 1.

Strains and plasmids used in this study (E. coli)

Strain or plasmid	Characters^a	Source
E. coli
DH5α	Sup E44 ΔlacU169 (ϕ 80lac ZΔM15) hsd R17 recAI endAI gyrA96 thi-1 relA	Laboratory stock
B. subtilis
B. subtilis WB600	B. subtilis gene-deficient strain (trpC2, ΔnprE, ΔaprE, ΔnprB, Δbpr, Δmpr, and Δepr)	Wu et al. (1991)
W	WB600Δupp	This work
Wm	WB600ΔuppΔsigF	This work
WA	WB600Δupp, hasA	This work
WmA	WB600ΔuppΔsigF, hasA	This work
WmB	WB600ΔuppΔsigF, hasA, hasB, and hasC	This work
1A	B. subtilis 168, hasA	This work
1B	B. subtilis 168, hasA, hasB, and hasC	This work
Plasmid
pCU	pUC18, Amp^R, Cm^R, B. subtilis counter-selectable upp integration vector	Fu et al. (2012)
pCU-sigF	sigF, pUC18, Amp^R, Cm^R, B. subtilis counter-selectable upp integration vector	This work
pHY300plk	Amp^R, Tet^R, E. coli–B. subtilis shuttle vector	Ishiwa and Tsuchida (1986)
pHY300plk-hasA	hasA, Amp^R, Tet^R, E. coli–B. subtilis shuttle vector	This work
pHY300plk-hasABC	hasA, hasB and hasC, Amp^R, Tet^R, E. coli–B. subtilis shuttle vector	This work

^aAmp^R: ampicillin resistance cassette; Cm^R: chloromycetin resistance cassette; Tet^R: tetracycline resistance cassette