Skip to main content
. 2019 May 21;9(6):225. doi: 10.1007/s13205-019-1749-x

Table 2.

Primers used in this study

Primer Sequence(5′–3′)a
upp-upF CGGGGTACCAGCGATGGCGTCTTATGC
upp-upR CGCCACAGTAATCATCTCGGTTTATGAGCGAGGG
upp-downF CCCTCGCTCATAAACCGAGATGATTACTGTGGCGAGTG
upp-downR GCTCTAGACGATACCCATGAACATAAGAG
sigF-upF GCTCTAGATACGGCTGAAACCCTGAA
sigF-upR GGAAACCTGCACCTGAGAAACCTCCACATCCATAACAA
sigF-downF TTGTTATGGATGTGGAGGTTTCTCAGGTGCAGGTTTCC
sigF-downR TCCCCCGGGTGGGCTCTTCATTTAGGC
hasA-F GCTCTAGAGTTTGTTGAACTAATGGGTG
hasA-R CCGGAATTCATAATGGTCGCAGTGTCG
hasABC-F1 ACGCGTCGACTATCACCGCCCAGCCTAA
hasABC-R1 GATCTTCCAATTTATAATGGTCGCAGTGTCGGTACC
hasABC-F2 GGTACCGACACTGCGACCATTATAAATTGGAAGATC
hasABC-R2 TGATCGAAAAATCTAGACAGCTTCAACCAAGTAACA
hasABC-F3 TGTTACTTGGTTGAAGCTGTCTAGATTTTTCGATCA
hasABC-R3 TCCCCCGGGTTTGGAAAGCGAGGGAAG
hasABC-VF AGTCGGAACGCCTATGTC
hasABC-VR TCACTTGTCAGCGGGTCA
qRT16S-F CGGTTTCGCTGCCCTTTGTT
qRT16S-R TGGGTTAAGTCCCGCAACGAG
qRTclsA-F GCCCGATCACGCCTTTGTAT
qRTclsA-R AATGTTCGCTGTGCCGACTG

aRestriction sites used for cloning are underlined