Table 2.
Primer | Sequence(5′–3′)a |
---|---|
upp-upF | CGGGGTACCAGCGATGGCGTCTTATGC |
upp-upR | CGCCACAGTAATCATCTCGGTTTATGAGCGAGGG |
upp-downF | CCCTCGCTCATAAACCGAGATGATTACTGTGGCGAGTG |
upp-downR | GCTCTAGACGATACCCATGAACATAAGAG |
sigF-upF | GCTCTAGATACGGCTGAAACCCTGAA |
sigF-upR | GGAAACCTGCACCTGAGAAACCTCCACATCCATAACAA |
sigF-downF | TTGTTATGGATGTGGAGGTTTCTCAGGTGCAGGTTTCC |
sigF-downR | TCCCCCGGGTGGGCTCTTCATTTAGGC |
hasA-F | GCTCTAGAGTTTGTTGAACTAATGGGTG |
hasA-R | CCGGAATTCATAATGGTCGCAGTGTCG |
hasABC-F1 | ACGCGTCGACTATCACCGCCCAGCCTAA |
hasABC-R1 | GATCTTCCAATTTATAATGGTCGCAGTGTCGGTACC |
hasABC-F2 | GGTACCGACACTGCGACCATTATAAATTGGAAGATC |
hasABC-R2 | TGATCGAAAAATCTAGACAGCTTCAACCAAGTAACA |
hasABC-F3 | TGTTACTTGGTTGAAGCTGTCTAGATTTTTCGATCA |
hasABC-R3 | TCCCCCGGGTTTGGAAAGCGAGGGAAG |
hasABC-VF | AGTCGGAACGCCTATGTC |
hasABC-VR | TCACTTGTCAGCGGGTCA |
qRT16S-F | CGGTTTCGCTGCCCTTTGTT |
qRT16S-R | TGGGTTAAGTCCCGCAACGAG |
qRTclsA-F | GCCCGATCACGCCTTTGTAT |
qRTclsA-R | AATGTTCGCTGTGCCGACTG |
aRestriction sites used for cloning are underlined