Fig. 2.

At baseline, GSC markers, HA receptors, and drug resistance genes are highly co-expressed in GBM, with cell-line dependence. a Percent of population expressing SOX2 and CD44 within U87-MG and D456 cells treated in NBE with varying concentrations of HA for a full passage length, measured via flow cytometry (mean ± SE; n = 3). b Confocal microscopy was performed on D456 cells grown in NBE for NANOG, SOX2, and HYAL1 (scale bars = 200 μm). c qRT-PCR gene expression of (left to right) GSC markers, HA-related genes, and drug resistance genes in U87-MG and D456 cells. Reported as relative expression compared to housekeeping gene GAPDH with log₁₀ transformation and significance noted between cell lines. HYAL1 and MDR1 were lower than detection level (n.d.) in D456 (mean ± SE; n = 3; * p < 0.05, † p < 0.01, and ‡ p < 0.001