Fig. 3.

HA promotes GSC sphere formation. a Representative micrographs of U87-MG and D456 cells treated with HA for a full passage length (scale bar = 100 μm). b qRT-PCR gene expression of U87-MG and D456 cells treated with HA for a full passage length. Represented as fold change compared to control cells. HYAL1 was not detected in D456 cells. The log₂ transforms of fold changes greater than 1 or less than 1 were considered significant (mean ± SE; n = 3). c ELDA of U87-MG and D456 cells treated without (“-HA”) or with HA (100 μg/mL; “+HA”) for 14 days (dotted lines 95% CI; n = 16; p < 0.05 indicates significant change in stem cell frequency)