Fig. 4.
HAase causes differentiated phenotype and attenuated growth in U87-MG cells, but not in D456 cells. a Representative micrographs of U87-MG and D456 cells treated with HAase for a full passage length. Arrowheads show adhered cells with spread morphology (scale bar = 100 μm). b Live cell counts of U87-MG and D456 cells treated with HAase for a full passage length. Dead population was determined with trypan blue staining prior to automatic cell counts (mean ± SE; n = 3; § p < 0.0001)