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HAase decreases stemness through involvement of CD44 signaling. qRT-PCR gene expression of U87-MG and D456 cells treated with HAase for a full passage length. a GSC markers CD133, SOX2, NES, and NANOG. b HA-related genes HAS2, RHAMM, and CD44. c Drug resistance genes STAT3, EGFR, and MDR1. MDR1 was not detected in D456 cells. All genes reported as relative expression compared to housekeeping gene GAPDH (mean ± SE; n = 3;* p < 0.05, † p < 0.01)
