Fig. 6.

HAase is cytotoxic to GBM cells and increases TMZ sensitivity in GSC-promoting culture. a Treatment of U87-MG cells grown in MEM media containing serum (+TMZ = 400 μM). b Treatment of U87-MG cells grown in GSC-promoting NBE media (+TMZ = 400 μM). c Treatment of D456 cells grown in GSC-promoting NBE media (+TMZ = 200 μM). Relative cell number was measured via a WST-8 assay after 48 h treatment with TMZ and/or HAase on Day 3 (+HAase = 15 U/mL; ++HAase = 100 U/mL). Both normalization and significance were determined relative to DMSO/PBS treated control wells (mean ± SE; n = 8; * p < 0.05, † p < 0.01, ‡ p < 0.001, and § p < 0.0001)