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. 2019 May 15;13:192. doi: 10.3389/fncel.2019.00192

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Copyright © 2019 Shi, Lin, Saliba, Xie, Neely and Banerjee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ringer and Futsch localization and genetic interactions. (A–C″) Confocal images showing localization of Futsch (green, A,A″,B,B″,C,C″), Ringer (red, A′,A″,B′,B″,C′,C″) and Hrp (blue, A″–C″) at NMJ of wild type (A–A″), ringer–/– (B–B″) and futsch^K68/K68 (C–C″). Representative confocal images showing the synaptic growth in ringer/+ (D), futsch^K68/+ (E), futsch^K68/+; ringer/+ (F), ringer–/– (G), futsch^K68/K68 (H), and futsch^K68/K68; ringer–/– (I) labeled with Dlg (green) and Hrp (red). Quantification of fluorescence intensity ratio of Ringer/Hrp (J) and Futsch/Hrp (K) and total Type 1 boutons (L) in specified genotypes. n = 15 animals per genotype, Error bars represent mean ± SEM [^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p < 0.001, not significant (ns)] (ANOVA).