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. 2019 May 15;13:192. doi: 10.3389/fncel.2019.00192

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Copyright © 2019 Shi, Lin, Saliba, Xie, Neely and Banerjee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reduced acetylated-tubulin levels in ringer and futsch mutant NMJs. (A–F″) Representative confocal images of NMJ branches showing Ac-Tub (green, A–F″) and Tub (red, A′–F″) localization in wild type (A–A″), ringer–/– (B–B″), futsch^K68/K68 (C–C″), futsch^K68/K68;ringer–/– (D–D″), elav-Gal4; UAS-ringer (E–E″), and elav-Gal4/UAS-Futsch[EP1419] (F–F″). (G) Quantification of the ratio of Ac-Tub/Tub fluorescence intensities in represented genotypes. n = 10 animals (20 NMJ branches analyzed/genotype). Error bars represent mean ± SEM [^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p < 0.001, not significant (ns)] (ANOVA). Scale bar: 5 μm.