Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 21;9:7666. doi: 10.1038/s41598-019-43948-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Interaction between Runx1 and Sox proteins. (a) Levels of Col2a1 mRNA, glycosaminoglycan (GAG), and Toluidine blue staining in C3H10T1/2 cells adenovirally transduced with Runx1 in combination with GFP, Sox5 + Sox6, Sox9, or Sox5 + Sox6 + Sox9. Data are expressed as means (bars) ± SD (error bars) of six samples per group *P < 0.01 versus each Ax-GFP control. (b) Levels of Col10a1 mRNA in ATDC5 cells adenovirally transduced with Runx1 in combination with GFP, Sox5 + Sox6, Sox9, or Sox5 + Sox6 + Sox9. Cells were cultured in pellets for 3 days after adenoviral transduction. Data are expressed as means (bars) ± SD (error bars) of four samples per group *P < 0.01 versus each Ax-GFP control. (c) Co-immunoprecipitation (Co-IP) assay using cell lysates of human articular chondrocytes. Cell lysates underwent IP with an antibody to Flag or Runx1, and were then immunoblotted with the other antibody. Full-length blots are presented in Supplementary Figure 2. (d) Mammalian two-hybrid assay by transfection of luciferase reporter vectors expressing GAL4–Runx1 (FL, full-length Runx1; Runt, the runt domain only; and ΔRunt, Runx1 mutant lacking the runt domain) and VP16–Sox proteins with GAL4 binding sites into HuH-7 cells. Data are expressed as means (bars) ± s.d. (error bars) of four samples per group *P < 0.01 vs. FL. (e) Immunocytochemistry of endogenous Runx1, Sox5, Sox6, and Sox9 in human articular chondrocytes were detected by secondary antibodies with red and green fluorescence, respectively. Scale bar, 10 μm.