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. 2019 May 21;9:7665. doi: 10.1038/s41598-019-43492-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Translation of assay via matched fresh frozen and FFPE tissue. (a) A summary of the INCLIVA-Valencia cohort, showing patient characteristics and sample size. (b) A scatter plot showing median expression of the 38 genes in samples with tumour cellularity ≥70% in both fresh frozen and FFPE tissues (n = 24). Colours indicated each gene’s association with the subtypes. (c) Alluvial diagram showing the subtype classification of matched fresh frozen and FFPE tissues for cellularity-selected samples (excluding undetermined samples; n = 14). (d) A summary of the Singapore FFPE cohort, showing patient characteristics and sample size. (e) A heatmap showing the expression of the 38-gene panel in Singapore FFPE samples as measured using the NanoCRCA assay (n = 106). The subtypes as assigned using the NanoCRCA assay are shown on the top bar. The right-hand vertical bar indicates the subtype association of each gene. Subtype colours are as in b).