Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 15;10:1095. doi: 10.3389/fimmu.2019.01095

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Zhang, Fu, Wang, Guan, Zhu, Guo, Zheng and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Viral non-structural protein (NSs) contribute to miR-146b expression resulted in inducing M2 macrophage differentiation. (A) Western blot analysis of SFTSV non-structural protein (NSs) and miR-146a/b expression (B) in THP-1 cells that were transfected control pCMV3 plasmid, NSs over-expression plasmid (pCMV3-NSs) for 48 h, respectively. SFTSV-infected THP-1 cells as a positive control. (B,C) THP-1 cells were transfected pCMV3 plasmid or pCMV3-NSs for 48 h. The expression of miR-146a/b and endogenous STAT1 detected by real-time PCR and western blot, respectively. (D) Immunofluorescence staining analyzed for CD86 and CD206 expression after transfected with pCMV3 or pCMV3-NSs for 48 h. Images were acquired using a confocal microscope. Right graph: fluorescence intensity of CD86 (green) and CD206 (red) in the regions delineated by a white line through ImageJ software.