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. 2019 May 15;10:1077. doi: 10.3389/fimmu.2019.01077

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Copyright © 2019 Butler, Sinkora, Wang, Stepanova, Li and Cai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of lymphoid cells and B lymphocyte subsets from the bronchio-alveolar lavage following infections of isolator piglets with PRRSV VR-2332 strain and SIV. Cell suspensions were analyzed according to phenotype using flow cytometry. The CD2⁻CD21⁺ subpopulation is comprised of primed/activated while CD2⁺CD21⁻ are memory/plasma cells. The functional role of these subpopulations has been described (32, 33). Statistically significant difference from GF animals are indicated by asterisks.