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. 2019 May 14;6(2):ENEURO.0399-18.2019. doi: 10.1523/ENEURO.0399-18.2019

Figure 6.

Figure 6.

Inhibiting BMP4/BMPRI signaling in OPCs alters the expression of transcription factor Id4 and Gfap, but not Mbp or Myrf. A, B, qRT-PCR analysis of Id4 and Gfap transcript levels from OPCs cultured in differentiation media and treated with LDN-193189, BMP4, or both, or vehicle (control) over various time points. *BMP4 significantly increased the level of Id4 transcripts at 2 and 24 h compared with the control, and this upregulation is blocked by pretreatment with LDN-193189 before BMP4 exposure. *Gfap expression was also significantly reduced by pretreatment of OPCs with LDN-193189 before BMP4 exposure. C, D, qRT-PCR analysis of myelin protein gene Mbp and key myelination transcription factor Myrf from OPCs treated with LDN-193189, BMP4, or both, or vehicle over 24 h. BMP4 significantly reduced the expression level of both Mbp and Myrf genes, with this effect reduced by LDN-193189 pretreatment; N = 3 independent cultures/group). *p < 0.05, **p < 0.01.