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. 2019 May 21;10(3):e00326-19. doi: 10.1128/mBio.00326-19

FIG 6.

FIG 6

Transcriptional analysis of glnA4 in the presence of ammonium, polyamines, and ethanolamine as sole nitrogen sources. Data represent results of reverse transcriptase PCR analysis of glnA4 and hrdB (control) from S. coelicolor M145 cultivated in defined Evans medium with 5 mM (Am-) or 50 mM (Am+) concentrations of ammonium chloride, polyamines (Put, putrescine; Cad, cadaverine; Spd, spermidine, 25 mM each) or ethanolamine (EtAm; 25 mM) and glucose as the sole carbon source at a high concentration (Glc+; 25 g/liter) or a low concentration (Glc-; 2.5 g/liter). Total RNA was isolated from mycelium harvested after 24 h of cultivation in defined Evans medium.