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HPLC/MS analysis of the GlnA4-catalyzed reaction. Samples were taken after 0 (A and C) and 10 min (B and D) and analyzed in −MS mode. Extracted ion chromatograms for glutamate with a mass-to-charge ratio of 146 m/z (A and B) and for gamma-glutamylethanolamide with 189 m/z (D) are shown. Intens, intensity. (C) In the absence of the GlnA4 no product (gamma-glutamylethanolamide) could be detected.
