FIG 3.
Quantitative analysis of global OMP levels. (A) The relative fold change of β-barrel OMPs comparing the ΔbamB ΔbamE, ΔbamB ΔbamE ΔrcsF, ΔbamB ΔbamE bamAF494L, and ΔbamB ΔbamE ΔrcsB mutants to the wild type is shown. Whiskers indicate 95% confidence intervals. The ΔbamB ΔbamE double mutant exhibits a universal defect in OMP levels with respect to the wild-type control. OMP levels are similarly reduced in the ΔbamB ΔbamE ΔrcsB triple mutant. Both ΔrcsF and bamAF494L restore OMP levels, to similar degrees. (B) Projecting the OMP protein abundance changes for various mutants on the vector defined by the differences between the wild type and ΔbamB ΔbamE double mutant indicates that ΔbamB ΔbamE ΔrcsB does not rescue the phenotype of the double mutant, but both ΔbamB ΔbamE ΔrcsF and ΔbamB ΔbamE bamAF494L partially rescue the OMP expression phenotype.