TABLE 1.
Primers and probes used in this study
| Organism | Gene | Primer name | Sequence 5′→3ʹ | Annealing temp (°C) | Product size (bp) | Reference or source |
|---|---|---|---|---|---|---|
| Borrelia spp. | 23S rRNA | Bor-1 | AGAAGTGCTGGAGTCGA | 53 | 260 | 40 |
| Bor-2 | TAGTGCTCTACCTCTATTAA | |||||
| Bor-3 | GCGAAAGCGAGTCTTAAAAGG | 58 | 222 | |||
| Bor-4 | ACTAAAATAAGGCTGAACTTAAAT | |||||
| Borrelia spp. | rrs-rrlA IGS | Bb IGS-F | GTATGTTTAGTGAGGGGGGTG | 56 | Different for different species | 41 |
| Bb IGS-R | GGATCATAGCTCAGGTGGTTAG | |||||
| Bb IGS-Fn | AGGGGGGTGAAGTCGTAACAAG | 60 | ||||
| Bb IGS-Rn | GTCTGATAAACCTGAGGTCGGA | |||||
| Borrelia spp. | Flagellin | FlaF1A | AGCAAATTTAGGTGCTTTCCAA | 60 | 154 | 42 |
| FlaR1 | GCAATCATTGCCATTGCAGA | |||||
| Fla Probe1 | TGCTACAACCTCATCTGTCATTGTAGCATCTTTTATTTG | |||||
| Mus musculus | actin | Mmact-F | AGAGGGAAATCGTGCGTGAC | 60 | 137 | 43 |
| Mmact-R | CAATAGTGATGACCTGGCCGT | |||||
| Mmact-P | CACTGCCGCATCCTCTTCCTCCC | |||||
| Borrelia afzelii | ospA | RTospA-F | GGTTCTGGAGTGCTTGAAGG | 55 | 112 | 45 |
| RTospA-R | TGTTTTGCCATCTTCTTTG | |||||
| Borrelia afzelii | bbk32 | RTbbk32-F | CACGTCTTGACAACCTTGCT | 55 | 117 | |
| RTbbk32-R | CCTTGCACTCACTTGAATATAG | |||||
| Borrelia afzelii | flaB | RTflaB-F | GTTCATGTGGGAGCAAATCA | 55 | 120 | |
| RTflaB-R | ACCCTCTTGAACAGGTGCAG | |||||
| Borrelia afzelii | ospC | BaospC-F | GCAGGAGCCTATGCAATATCA | 60 | 150 | This study |
| BaospC-R | TTTGCCAAGATCTGCATGAC |