FIG 3.

The three novel LEDs bind specifically to PI3P in vitro. Affinity-purified GST fusion proteins (150 pmol) of SidC and GST controls (A), LED006 from LegC6, lpg2327, Lem9, LegC5, Ceg22, LepB, or LegK1 (B), LED027 from Ceg19 or lpg1961 (C), and LED035 from RavB, Lem21, or MavH (D) were assayed for binding to different synthetic lipids (100 pmol) immobilized to nitrocellulose membranes by a protein-lipid overlay assay using an anti-GST antibody. The different lipids immobilized to the strip are indicated in panel A. PI3P, phosphatidylinositol 3-phosphate.