TABLE 1.

PI4P- and PI3P-binding L. pneumophila effectors

Locus taga	Name	Size (aa)	Position of PI-BD (aa)b	PI bindingc	Localizationd	Effector function	Reference(s)
lpg0160	RavD	325	190–325	PI3P	LCV	Helps to prevent encounters of the LCV with lysosomes	59
lpg0695	AnkX	949	491–949	PI3P, PI4P	LCV	Phosphocholinates Rab1 and Rab35	60–64
lpg0940	LidA	729	60–180	PI3P, PI4P	LCV	Modulates Rab1 to render it persistently active	33, 65–69
lpg1101	Lem4	322	201–322	PI4P	LCV	Phosphotyrosine phosphatase	70, 71
lpg1683	RavZ	502	329–423	PI3P	AP	Irreversible deconjugation of Atg8/LC3	40, 42, 72
lpg1978	SetA	644	401–644	PI3P	LCV	Glucosyltransferase, subverts vesicle trafficking	73, 74
lpg2222	LpnE	375	ND	PI3P	LCV	Binds polyphosphate 5-phosphatase OCRL1	75–77
lpg2311	RidL	1,167	867–1167	PI3P	LCV	Binds the retromer subunit Vps29 and inhibits retrograde trafficking	78–81
lpg2464	SidM/DrrA	647	544–647	PI4P	LCV	AMPylates Rab1 and Rab35, activates Rab1	33, 82–85
lpg2510	SdcA	908	605–771	PI4P	LCV	E3 ubiquitin ligase, recruits ER to the LCV	32
lpg2511	SidC	917	609–776	PI4P	LCV	E3 ubiquitin ligase, recruits ER to the LCV	32, 43, 86–90
lpg2603	Lem28	434	326–434	PI4P	LCV	ND	70
lpw03701	LtpD	679	472–626	PI3P	LCV	Binds a phosphatase involved in PI production	91
lpp0356	LtpM	639	469–639	PI3P	LCV	Glycosyltransferase	92

^alpp0356 is found in L. pneumophila strain Paris, and lpw03701 is found in L. pneumophila strain 130b and not in L. pneumophila strain Philadelphia-1.

^bPI-BD, phosphoinositide-binding domain; ND, not determined.

^cPI3P, phosphatidylinositol 3-phosphate; PI4P, phosphatidylinositol 4-phosphate.

^dLCV, Legionella-containing vacuole; AP, autophagosomes.