FIG 2.

Assessment of anti-PfCelTOS IgG subclass profiles and longevity. Preimmune sera (n = 30) were used as negative controls to determine the ELISA cutoffs, which were calculated as the mean OD₄₉₀ plus 3 SD. The cutoffs for total IgG (TIgG), IgG1, IgG2a, IgG2b, and IgG3 were OD₄₉₀ values of 0.101, 0.064, 0.072, 0.093, and 0.091, respectively, on day 38 and 0.1, 0.083, 0.089, 0.108, and 0.068, respectively, on day 180 after the first immunization. The bars and error bars show the mean OD₄₉₀ values and standard deviations (SD) for 16 individual mice (day 38) and 10 individual mice (day 180) in each group, respectively. The highest levels of total IgG, IgG2a, and IgG2b were detected in group 5, which received PfCelTOS in combination with MCQ (MPL/CpG/QS-21) adjuvants on days 38 and 180 after the first immunization (P < 0.05 by one-way ANOVA). The table shows multiple comparisons of means for anti-PfCelTOS IgG and its subclasses among the nonadjuvanted (group 1) and adjuvanted (groups 2 to 5) vaccine groups on days 38 and 180 after the first immunization, which were performed by using Tukey’s HSD post hoc test.