FIG 3.

Evaluation of endpoint titers of anti-PfCelTOS total IgG, IgG2a, and IgG2b antibodies. For titration, 1:200 to 1:409,600 dilutions of mouse sera from different vaccine groups (groups 1 to 5) were analyzed using an ELISA. The bars show the last dilution of test sera in which OD₄₉₀ values were above that of cutoff. Among all the vaccine groups (groups 1 to 5), the highest endpoint titers of anti-PfCelTOS total IgG, IgG2a, and IgG2b were detected in mouse group 5 receiving PfCelTOS/MCQ (MPL/CpG/QS-21) on days 38 and 180 after the first immunization. The cutoffs for total IgG, IgG2a, and IgG2b were OD₄₉₀ values of 0.1, 0.072, and 0.093, respectively, on day 38 and 0.1, 0.089, and 0.108, respectively, on day 180.