FIG 5.

Proliferation of lymphocytes in cultured splenocyte cells of immunized mice (groups 1 to 10) in the presence of PfCelTOS antigen in vitro. On days 38 and 180 after the first immunization, spleens from each mouse group (4 mice from each vaccine and control group) were removed, and lymphocyte proliferation was evaluated using the MTT assay. On day 180 after the primary immunization, lymphocyte proliferation was significantly reduced in all the mouse vaccine groups (P < 0.05 by a paired-sample t test). Data were analyzed using one-way ANOVA, followed by Tukey’s HSD post hoc test. MCQ, MPL/CpG/QS-21.