FIG 6.

Assessment of IFN-γ, TNF-α, and IL-10 production in vaccine groups (groups 1 to 5) and control groups (groups 6 to 10) on days 38 and 180 after the first immunization by an ELISA. (A) For immunized mice receiving PfCelTOS with different adjuvants alone or in combination, the mean IFN-γ responses in the presence of ConA (as the positive control) and no antigen (as the negative control) were in the range of 1,821 to 2,101 and 21 to 35 pg/ml, respectively. (B) Among different vaccine groups, the mean TNF-α responses in the presence of ConA (as the positive control) and no antigen (as the negative control) were in the range of 964 to 1,150 and 20 to 38 pg/ml, respectively. (C) IL-10 responses in the presence of ConA (as the positive control) and no antigen (as the negative control) were in the range of 152 to 459 and 19 to 35 pg/ml, respectively. On day 180 after primary immunization, the levels of all cytokines were significantly reduced in all the vaccine mouse groups (P < 0.05 by a paired-sample t test). The table shows multiple comparisons of IFN-γ, TNF-α, and IL-10 among the nonadjuvanted (group 1) and adjuvanted (groups 2 to 5) vaccine groups on days 38 and 180 after the first immunization, which was performed by using Tukey’s HSD post hoc test. MCQ, MPL/CpG/QS-21.