FIG 7.

Evaluation of Th1/Th2 ratios. Th1 (anti-PfCelTOS IgG2a and IgG2b antibodies and cytokines IFN-γ and TNF-α) and Th2 (anti-PfCelTOS IgG1 and the cytokine IL-10) responses were analyzed in different vaccine groups on days 38 and 180 after the first immunization. (A) For immunized mice receiving PfCelTOS alone or with different adjuvants (groups 1 to 5), an increase in the IgG2a/IgG1 ratio was observed in mouse group 5, which received PfCelTOS in combination with MCQ (MPL/CpG/QS-21), on day 38 (ratio, 2.76; P < 0.05 by one-way ANOVA) and day 180 (8.9). (B) In mouse group 5, which received the antigen with MCQ adjuvants, the IFN-γ/IL-10 ratios (day 38 ratio, 21.1; day 180 ratio, 24.12) were significantly higher than those in mouse group 1, which received the antigen alone (P < 0.05 by one-way ANOVA). The table shows multiple comparisons of IgG2a/IgG1, IgG2b/IgG1, IFN-γ/IL-10, and TNF-α/IL-10 (Th1/Th2) ratios among the nonadjuvanted (group 1) and adjuvanted (groups 2 to 5) vaccine groups on days 38 and 180 after the first immunization using Tukey’s HSD post hoc test.