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. 2019 Mar 23;64(3):209–216. doi: 10.3164/jcbn.18-112

Fig. 4.

Fig. 4

Changes induced by NUPR1 knockdown. (A) Western blot analysis in MSCs after siRNA-induced NUPR1 knockdown. (B) Effects of NUPR1 knockdown on MSC proliferation. Proliferation was measured every 2 h with IncuCyte (n = 6). (C) Effects of NUPR1 knockdown on staurosporine-induced apoptosis. Caspase-3 activity is shown 6 h after treatment with staurosporine was shown. Three thousand cells were seeded in 96-well plates and Caspase-3 activity was measured 6 h after staurosporine treatment. Caspase activity was normalized based on the cell number deduced from analysis with CyQUANT® cell proliferation assay. * indicates statistically significant difference compared with siCONTROL #1, p<0.01.