Table 4.

CCD Experimental design developed using Design Experts STAT EASE Software.

Std	A	B	C	D	Uricase activity (U/ml)
					Observed	Predicted
1	−1	−1	−1	−1	15.83	15.23
2	+1	−1	−1	−1	6.99	6.57
3	−1	+1	−1	−1	15.68	15.23
4	+1	+1	−1	−1	10.69	10.22
5	−1	−1	+1	−1	11.17	10.66
6	+1	+1	+1	−1	6.85	7.25
7	−1	+1	+1	−1	5.2	5.33
8	+1	−1	+1	−1	15.45	15.23
9	−1	+1	−1	+1	10.85	10.13
10	+1	+1	−1	+1	6.11	6.38
11	−1	−1	−1	+1	10.98	12.19
12	+1	+1	−1	+1	7.35	6.71
13	−1	−1	+1	+1	15.63	15.23
14	+1	−1	+1	+1	15.75	15.23
15	−1	+1	+1	+1	13.9	14.15
16	+1	+1	+1	+1	10.72	12.09
17	−1.68	0	0	0	14.14	14.59
18	+1.68	0	0	0	5.6	5.94
19	0	−1.68	0	0	11.24	11.16
20	0	+1.68	0	0	4.16	4.10
21	0	0	−1.68	0	10.11	9.44
22	0	0	+1.68	0	9.5	9.09
23	0	0	0	−1.68	14.42	15.23
24	0	0	0	+1.68	4.73	5.24
25	0	0	0	0	7.35	7.86
26	0	0	0	0	5.38	5.84
27	0	0	0	0	9.84	9.17
28	0	0	0	0	10.83	11.95
29	0	0	0	0	7.33	6.71
30	0	0	0	0	12.125	11.78