Figure 5.

NPC1-Specific ASO 05HM Did Not Stimulate Cytokine Release from Treated PBMCs

(A–C) PBMCs from three different donors were treated with oligonucleotides and immune stimulatory agents (each condition in triplicate wells), with the indicated concentrations. The third day after treatment, supernatants were harvested and used for determination of cytokine release using ELISA: (A) IFNγ, (B) IL6, and (C) TNFα. Values were displayed as fold changes compared to untreated. Means and SDs were also indicated. ANOVA test was used to test for significant differences and p values were determined using Dunnett’s test in GraphPad Prism 7.04 Software.