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. 2019 Apr 23;16:675–685. doi: 10.1016/j.omtn.2019.04.017

Figure 1.

Figure 1

miR-150-5p Was Significantly Suppressed in Cancer Stem Cells

(A) Heatmap showing miRNAs that were downregulated by more than 1.5-fold in ALDH-positive and CD133-high-expression cells compared to ALDH-negative and CDD133-negative cells, respectively. ALDH-positive and -negative or CDD133-high-expression and -negative cells were separated from A549 cells by flow cytometry sorting and were then subjected to miRNA array assay. (B) miRNAs were simultaneously downregulated by more than 2-fold in both ALDH-positive and CD133-high-expression cells compared to ALDH-negative and CDD133-negative cells, respectively. (C) qRT-PCR analysis showed that miR-150-5p was significantly suppressed in sphere-formed NSCLC cells compared to cells that grew adherently. (D) qRT-PCR analysis showed that miR-150-5p was significantly downregulated in CD133-high-expression cells compared to CD133-negative cells from NSCLC patients (n = 3).