Figure 2.

Inhibition of miR-150-5p Enhanced NSCLC Cell Stemness and Metastatic Colonization

(A) Inhibition of miR-150-5p significantly decreased ALDH-positive cells in NSCLC cells. The indicated cells were infected with miR-150-5p antisense-expressing lentivirus. At 72 h after infection, the cells were subjected to flow cytometry. (B) Inhibition of miR-150-5p significantly enhanced sphere formation in NSCLC cells. (C) Inhibition of miR-150-5p enhanced the tumorigenicity of A549 cells. A total of 5 × 10³ or 5 × 10⁴ miR-150-5p antisense-expressing A549 cells (left back) and vector control cells (right back) was subcutaneously injected into nude mice (n = 5). (D) Inhibition of miR-150-5p stimulated H1299 cell metastatic colony formation in lungs. miR-150-5p antisense-expressing A549 cells or control cells were injected into nude mice through the tail vein (n = 5). At 1 month after cell injection, mice were sacrificed and tumor colony numbers were counted on the lung surface. The histological section was stained with H&E.