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. 2019 May 15;10:578. doi: 10.3389/fphys.2019.00578

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Copyright © 2019 Freitas Leal, Preijers, Brock, Adjobo-Hermans and Bosman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RBCs morphology and phenotype of PNH patients. (A) Percentage of discocytes per Percoll fraction in PNH patients (N = 5) and healthy control donors (N = 5); (B) bright field and fluorescence images of anti-CD59-Alexa 647 stained RBCs from a healthy subject and a PNH patient, showing CD59 density; (C) RBCs of PNH patients (N = 9) were separated according to density and analyzed by flow cytometry regarding their CD59 content (type I, II, and III); (D) RBCs of PNH patients being treated with eculizumab (T; N = 5) and non-treated PNH patients (NT; N = 4) separated according to density and analyzed by flow cytometry according to their CD59 content (type I, II, and III). ^#Significantly different from type I in the same Percoll fraction (p < 0.05). U, unseparated; f1, f3, f5, fractions of increasing density isolated by Percoll density separation (Materials and Methods).