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. 2019 May 15;10:578. doi: 10.3389/fphys.2019.00578

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Copyright © 2019 Freitas Leal, Preijers, Brock, Adjobo-Hermans and Bosman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Complement deposition on density-separated RBCs. (A) Percentage of C3c-positive RBCs in the PNH RBC population divided per CD59 content (type I, II, and III) per density (Percoll fraction; N = 2); (B) percentage of C3d-positive RBCs in the PNH RBC population according to CD59 content (type I, II, and III) per Percoll fraction (type I, II, and III; N = 3). The samples were analyzed as described before (see Materials and Methods). U, unseparated; f1, f3, f5, fractions of increasing density isolated by Percoll density separation (Materials and Methods).