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. 2019 May 15;13:414. doi: 10.3389/fnins.2019.00414

FIGURE 2.

FIGURE 2

Afobazole prevents upregulation of the pro-apoptotic gene product, Bax, observed in response to ischemia in microglia. Microglia were double labeled with DAPI (blue) and anti-Bax antibody (Alexa Fluor 488 conjugated secondary antibody, green). (A) Photomicrographs of merged images of cultured microglia exposed for 24 h to media alone (i) or media containing 4 mM Azide (Isch) (ii), 30 μM afobazole (iii), or 4 mM Azide + 30 μM afobazole (iv). Fields of view shown are representative regions within larger images used to calculate number of Bax-positive cells. Scale bar in (i) is 10 μm. (B) Bar graph of mean number of Bax-positive microglia in experiments in which the cells were exposed to normal conditions (Control) or ischemia (Isch) in the absence (Media) or presence of 30 μM afobazole (Afob) (n = 9). Asterisks indicate significant difference between Control and Isch within Media and Afob, respectively (p < 0.001), and pound symbols denote significant difference between Media and Afob within Isch (p < 0.001).