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. 2019 May 15;13:414. doi: 10.3389/fnins.2019.00414

FIGURE 4.

FIGURE 4

Afobazole enhances Bcl-2 expression under control conditions and following ischemia in microglia. Microglia were double labeled with DAPI (blue) and anti-Bcl-2 antibody (Alexa Fluor 488 conjugated secondary antibody, green). (A) Photomicrographs of merged images of cultured microglia exposed for 24 h to media alone (i) or media containing 4 mM Azide (ii), 30 μM afobazole (iii) or 4 mM Azide + 30 μM afobazole (iv). Areas shown are representative regions within larger images used to calculate Bcl-2 expression. Scale bar in (i) is 10 μm. (B) Bar graph of mean percentage of Bcl-2-positive microglia in experiments in which the cells were exposed to normal conditions (Control) or ischemia (Isch), in the absence (Media) and presence of 30 μM afobazole (Afob) (n = 9). Asterisks indicate significant difference between Control and Isch within Media (p < 0.001), and pound symbols denote significant difference between Media and Afob within Control and Isch, respectively (p < 0.001).