FIGURE 6.

Afobazole fails to prevent upregulation of the pro-apoptotic gene product, Bax, observed in response to extended ischemia in neurons. (A) Photomicrographs of merged images of cultured neurons exposed for 24 h to media alone (i), or media containing 4 mM Azide (ii), 100 μM afobazole (Afob) (iii) or 4 mM Azide + 100 μM afobazole (Isch + Afob) (iv). Neurons were double labeled with DAPI (blue) and anti-Bax antibody (green). Fields of view shown are representative regions within larger images used to calculate Bax expression. Scale bar in (i) is 10 μm. (B) Bar graph of mean percentage of Bax-positive neurons in experiments in which the cells were exposed to the same conditions as (A) (n = 9). Asterisks indicate significant difference between Control and Isch within Media and Afob, respectively (p < 0.001).