FIGURE 9.

Afobazole fails to preserve neuronal membrane responses to elevated external K⁺ after prolonged ischemia. Bar graphs of mean change in depolarization-induced increase in Peak [Ca²⁺]_i (A) and total net calcium influx (Area) (B) evoked by application of high K⁺ (30 mM KCl) in isolated cortical neurons. Neurons were incubated for 24 h with afobazole alone (Media) or under ischemic conditions with 100 μM afobazole (Isch + Afob). Ischemic solution and afobazole were removed and neurons then incubated in normal media during a 72 h recovery (n ≥ 79). Asterisks indicate significant difference between Media and Isch + Afob (p < 0.001).