Figure 2.

2-AG increases the frequency of spontaneous reversals. Wild-type and mutant animals were incubated in 2-AG, and spontaneous reversals were assayed after 5 min on NGM plates containing 32 μm 2-AG, as described in Materials and Methods. The mutant animals used were as follows: cat-2(n4547), npr-19(ok2068), tph-1(mg280), 5-HT quint-null [ser-5(tm2654); ser-4(ok512); mod-1(ok103); ser-7(tm1325), ser-1(ok345)], mod-1(ok103), ser-1(ok345), and ser-4(ok512). * Indicates significantly different from wild-type animals at 5 min; † indicates significantly different from the same animals at time 0 (p ≤ 0.05). Data are presented as the mean ± SE (n) and were analyzed by two-tailed Student's t test using GraphPad software.