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. 2019 May 22;39(21):4142–4152. doi: 10.1523/JNEUROSCI.2371-18.2019

Figure 5.

Figure 5.

2-AG-dependent locomotory inhibition requires the TRP-like channels OSM-9 and TRP-4 in the serotonergic and dopaminergic neurons, respectively. Animals were incubated in 2-AG and locomotion was assayed as body bends/20 s as described in Materials and Methods. a, osm-9 (ky10)- and trp-4 (sy695)-null animals are resistant to 2-AG-dependent locomotory inhibition and the temporal patterns of resistance to 2-AG-dependent locomotory inhibition in osm-9- and trp-4-null animals mimics similar patterns in tph-1 (mg280)- and cat-2 (n4547)-null animals, respectively. b, Locomotion in trp-4- and cat-2-null animals is stimulated in the presence of 2-AG. c, Selective osm-9 and trp-4 RNAi knockdown in the serotonergic and dopaminergic neurons of wild-type animals, driven by the tph-1 and cat-2 promoters, respectively, abolishes 2-AG-dependent locomotory inhibition. Data are presented as the mean ± SE (n) and analyzed by two-tailed Student's t test using GraphPad software.