Figure 6.

Glutamatergic, GABAergic, and glycinergic neurons in the sacral cord of intact and spinal cord injured, antibody-treated rats at 28 d after injury. a–e, Representative images of fluorescent in situ hybridization for mRNA of GlyT₂ (a), GAD₂ (b), and vGlut₂ (c). d, Sections were counterstained with DAPI. e, Representative image of the three merged probes, indicating the laminae in the three analyzed regions (I–III; IV, V; X). f–h, Quantification of glycinergic (f), GABAergic (g), and glutamatergic (h) neurons in the sacral cord of intact animals, incomplete spinal cord-injured animals treated with control antibody (Ctr Ab), and incomplete spinal cord-injured animals treated with anti-Nogo-A antibodies (anti-Nogo-A). Data are presented as means ± SD. Scale bars: a–d, 20 μm; e, 300 μm; ns, not significant; *p < 0.05.